CRC 1208 Lecture
on Thursday, 28.04.2022,
4:30 pm- 6:00 p.m.
for all members of CRC 1208 and guests.

Meeting-Link: https://hhu.webex.com/hhu/j.php?MTID=m3d4ad28220e99a516597985291e9e52d

Meeting-Kennnummer:2731 742 2894  Passwort: cFGCkZ5EW83

Phill Stansfeld, University of Warwick, UK
"Molecular Modelling the Biogenesis of the Bacterial Cell Envelope"

Membrane proteins are bound and regulated by phospholipids and sterols from the surrounding bilayer and therefore determining how the nature of these interactions is vital for a full understanding of biological function. Molecular dynamics (MD) simulations, especially using coarse-grained (CG) methods, are routinely used to identify these binding sites. We are increasingly interested in determining not only if and where a particular lipid binds to a protein of interest, but also what the affinity of this interaction is and whether there is selectivity to the lipid binding. In other words, how likely is a specific protein-lipid interaction, given the complex environment of a biological membrane? I will discuss our methods to measure binding kinetics to identify and rank 700 cardiolipin binding sites on 42 Escherichia coli membrane proteins. This comprehensive approach enables identification of conserved motifs for high affinity cardiolipin-protein interactions. I will also discuss our studies into modelling mycobacterial membranes and approaches to determine how a bacterial cell establishes both its peptidoglycan cell wall and outer membrane.

Host: Birgit Strodel